RESEARCH PAPER
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 14-20

Indocyanine green-001 (ICG-001) attenuates wnt/β-catenin-induces myocardial injury following sepsis


Department of Pharmacology and Therapeutics, College of Medicine, University of Kufa, Kufa, Iraq

Correspondence Address:
Nasser Ghaly Yousif
Medical College, Al.Muthanna University, Samawah
Iraq
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jpp.JPP_153_16

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Objective: To investigate the mechanistic pathway of both indocyanine green (ICG)-001 in attenuated endotoxemia-induced cardiac depression through downregulation cardiac Wnt/ β-catenin cell signaling. Materials and Methods: Adult (4–6 months) male Albino-Webster mice, their weights ranged from 25 to 30 g, were pretreated with ICG-001 i.p., following cecal ligation and puncture (CLP). Left ventricle (LV) function was assessed using a microcatheter system. Monocyte chemoattractant protein-1 (MCP-1) and cytokines mediators in plasma and myocardium were analyzed by enzyme-linked immunosorbent assay. Further, the cardiac Wnt protein measured by quantitative real-time polymerase chain reaction while β-catenin analysis through Western blotting procedure. The pathological changes and cells injury in the myocardium were examined using hematoxylin and eosin staining. Results: CLP mice displayed worse LV function. The exaggerated cardiac depression in CLP mice was associated with higher levels of MCP-1 and cytokines in plasma and myocardium together with greater cardiac levels of cardiac troponin-I and Wnt/β-catenin. Neutralization of sepsis by either ICG-001resulted in improved LV function and reductions in inflammatory mediators. Conclusion: Taken together, these data showed that ICG-001 improved LV function following sepsis through downregulation of Wnt/β-catenin and serve as a potential mechanistic pathway ICG-001 in therapeutic cardiac endotoxemia in animal model.


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